Abstract | BACKGROUND: METHODS: To explore the role of ANT in DKD, we performed the examination of blood glucose, renal function, and histopathology. As for the mechanism, we designed the label-free quantification proteomics and nontargeted metabolomics analysis for kidney and serum. Subsequently, we revealed the anti-DKD effect of ANT through the bioinformatic analysis. RESULTS: We showed that the fasting blood glucose level (- 6.1 mmol/L, P = 0.037), perimeter of glomerular lesions (- 24.1 μm, P = 0.030), fibrosis score of glomerular (- 8.8%, P = 0.002), and kidney function ( Cystatin C: - 701.4 pg/mL, P = 0.043; urine creatinine: - 701.4 mmol/L, P = 0.032) were significantly alleviated in DKD mice after ANT treatment compared to untreated in the 20th week. Further, proteins and metabolites in the kidneys of DKD mice were observed to be dramatically altered due to changes in amino acid metabolism with ANT treatment; mainly, taurine and hypotaurine metabolism pathway was upregulated (P = 0.0001, t value = 5.97). Furthermore, upregulated tryptophan metabolism (P < 0.0001, t value = 5.94) and tyrosine metabolism (P = 0.0037, t value = 2.91) pathways had effects on serum of DKD mice as responsed ANT regulating. CONCLUSIONS: Our results suggested that prevention of the progression of DKD by ANT could be related to the regulation of amino acid metabolism. The use of dietary ANT may be one of the dietary strategies to prevent and treat DKD.
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Authors | Yi-Xi Li, Yong-Ping Lu, Donge Tang, Bo Hu, Ze-Yu Zhang, Hong-Wei Wu, Li-Jing Fan, Kai-Wen Cai, Chun Tang, Yi-Qing Zhang, Ling Hong, Jing-Jing Dong, Bao-Zhang Guan, Liang-Hong Yin, Yong Dai, Wei-Bin Bai, Zhi-Hua Zheng, Ting Zhu |
Journal | Journal of translational medicine
(J Transl Med)
Vol. 20
Issue 1
Pg. 510
(11 05 2022)
ISSN: 1479-5876 [Electronic] England |
PMID | 36335368
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Anthocyanins
- Blood Glucose
- Amino Acids
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Topics |
- Mice
- Animals
- Diabetic Nephropathies
(metabolism)
- Anthocyanins
(pharmacology, therapeutic use)
- Blood Glucose
- Kidney
(pathology)
- Amino Acids
- Diabetes Mellitus
(pathology)
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