Abstract | BACKGROUND: METHOD: The expression of FPR2 and nuclear factor-κB (NF-κB) signaling pathway was examined by real-time PCR, western blots, and analyzed via one-way analysis of variance. The distribution of FPR2 was detected using immunostaining. The expression of resolvin D1 (RvD1, the endogenous ligand of FPR2) was observed via enzyme-linked immunosorbent assay. Pearson's correlation test was used to evaluate the correlation between the expression levels of FPR2 and RvD1 and the clinical variants. RESULTS: The expression of FPR2 was significantly lower in FCDIIb (p = .0146) and TSC (p = .0006) cortical lesions than in controls, as was the expression of RvD1 (FCDIIb: p = .00431; TSC: p = .0439). Weak FPR2 immunoreactivity was observed in dysmorphic neurons (DNs), balloon cells (BCs), and giant cells (GCs) in FCDIIb and TSC tissues. Moreover, FPR2 was mainly distributed in dysplastic neurons; it was sparse in microglia and nearly absent in astrocytes. The NF-κB pathway was significantly activated in patients with FCDIIb and TSC, and the protein level of NF-κB was negatively correlated with the protein level of FPR2 (FCDIIb: p = .00395; TSC: p = .0399). In addition, the protein level of FPR2 was negatively correlated with seizure frequency in FCDIIb (p = .0434) and TSC (p = .0351) patients. CONCLUSION: In summary, these results showed that the expression and specific distribution of FPR2 may be involved in epilepsy caused by FCDIIb and TSC, indicating that downregulation of FPR2 mediated the dysfunction of neuroinflammation resolution in FCDIIb and TSC.
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Authors | Kaixuan Huang, Zhongke Wang, Zeng He, Yang Li, Shujing Li, Kaifeng Shen, Gang Zhu, Zhonghong Liu, Shengqing Lv, Chunqing Zhang, Hui Yang, Xiaolin Yang, Shiyong Liu |
Journal | Immunity, inflammation and disease
(Immun Inflamm Dis)
Vol. 10
Issue 11
Pg. e706
(11 2022)
ISSN: 2050-4527 [Electronic] England |
PMID | 36301030
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. |
Chemical References |
- FPR2 protein, human
- NF-kappa B
- Receptors, Formyl Peptide
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Topics |
- Humans
- Cerebral Cortex
(metabolism, pathology)
- Epilepsy
(genetics, metabolism)
- Inflammation
(pathology)
- Malformations of Cortical Development
(metabolism, pathology)
- NF-kappa B
(metabolism)
- Receptors, Formyl Peptide
(genetics, metabolism)
- Tuberous Sclerosis
(genetics, complications, metabolism)
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