Viral RNA extraction using an automatic nucleic acid extractor with magnetic particles and genetic characterization of bovine viral diarrhea virus in Tokachi Province, Japan, in 2016-2017.

Abstract

In this study, the viral genome extraction performance of automatic nucleic acid extractors and manual nucleic acid extraction kits was compared. We showed that compared with manual kits, the automatic extractors showed superior genome extraction performance using bovine viral diarrhea virus (BVDV) genome-positive cattle sera and bovine coronavirus/infectious bovine rhinotracheitis virus-spiked cattle nasal swabs. In addition, the subgenotyping of BVDV strains detected in Tokachi Province in Japan during 2016-2017 was performed. Results showed that most of these BVDV strains belonged to subgenotype 1b, while few strains belonged to subgenotypes 1a and 2a. This study showed the high applicability of automatic nucleic acid extractors in extracting multiple viral genomes and the dominant subgenotype of BVDV in Tokachi.

• Authors: Hieu Van Dong, Maya Suzuki, Hitoshi Takemae, Dulamjav Jamsransuren, Sachiko Matsuda, Hiep Dinh Nguyen, Tetsuya Mizutani, Yohei Takeda, Haruko Ogawa
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 84 Issue 11 Pg. 1543-1550 (Nov 14 2022) ISSN: 1347-7439 [Electronic] Japan
• PMID: 36216530 (Publication Type: Journal Article)

Chemical References

• RNA, Viral
• Nucleic Acids

Topics

• Cattle
• Animals
• Bovine Virus Diarrhea-Mucosal Disease
• RNA, Viral (genetics)
• Nucleic Acids
• Japan
• Genotype
• Diarrhea Viruses, Bovine Viral (genetics)
• Diarrhea (veterinary)
• Magnetic Phenomena
• Diarrhea Virus 1, Bovine Viral (genetics)
• Phylogeny
• Cattle Diseases