Differential detection of IgM and IgG antibodies to chimeric antigens in bovine tuberculosis.

Abstract

Recent studies have suggested the potential of innovative serologic tests for accurate and rapid detection of bovine tuberculosis (bTB). Dual Path Platform (DPP) technology has been used to develop rapid animal-side antibody tests for Mycobacterium bovis infection in a range of livestock and wildlife host species. The present study evaluated diagnostic performance of DPP BovidTB IgM/IgG assay designed for differential detection of bovine IgM and IgG antibodies against two chimeric antigens, DID38 and TBf2, respectively, using 662 well-characterized serum samples from M. bovis-infected and bTB-free cattle collected in the United States, Great Britain, France, and South Africa. Test sensitivity and specificity ranged from 71% to 100% and from 95% to 100%, respectively, depending on the country, with overall accuracy of 83%. No significant risk of cross-reactivity with serum samples from cattle infected with most relevant species of mycobacteria other than M. bovis was found. The DPP BovidTB IgM/IgG assay may be suitable for use in multi-test algorithms to improve current strategies for bTB surveillance.

• Authors: Archana A Sridhara, Ashley Johnathan-Lee, Rubyat Elahi, Paul Lambotte, Javan Esfandiari, Maria Laura Boschiroli, Tanya J Kerr, Michele A Miller, Thomas Holder, Gareth Jones, H Martin Vordermeier, Breanne N Marpe, Tyler C Thacker, Mitchell V Palmer, W Ray Waters, Konstantin P Lyashchenko
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 253 Pg. 110499 (Nov 2022) ISSN: 1873-2534 [Electronic] Netherlands
• PMID: 36215871 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Immunoglobulin G
• Immunoglobulin M

Topics

• Cattle
• Animals
• Tuberculosis, Bovine (diagnosis)
• Immunoglobulin G
• Mycobacterium bovis
• Serologic Tests (veterinary)
• Immunoglobulin M
• Cattle Diseases (diagnosis)