miR-30c functions as a tumor suppressor gene in the majority of
tumors, including
gliomas. In our study, we discovered that the expression levels of miR-30c in
glioma tissues and plasma prior to surgery were lower than those in normal brain tissue following
brain injury decompression and in plasma in healthy volunteers. The low expression of miR-30c was closely aligned with the WHO grade,
tumor size, PFS, and OS. Additionally, the miR-30c expression level in
tumor tissue was positively correlated with the levels in preoperative plasma. In cell biology experiments, miR-30c inhibited EMT and proliferation, migration, and invasion of
glioma cells. Analysis of databases of
miRNA target genes, real-time quantitative PCR, western blotting, and dual
luciferase reporter assays demonstrated that Notch1 is the direct target gene of miR-30c. An inhibitor and shRNA-Notch1 were cotransfected into
glioma cells, and it was found that shRNA-Notch1 reduced the enhancement of inhibitors of EMT and proliferation, migration, and invasion of
glioma cells. Therefore, we believe that when utilized as a tumor suppressor gene, miR-30c can inhibit EMT and the proliferation, migration, and invasion of
glioma cells by directly acting on Notch1 at the posttranscriptional level and that it is a potential diagnostic and prognostic marker.