Two trials were carried out to assess the effects of
arginine supplementation through ratios of digestible
arginine:lysine on growth performance, skin quality and
creatine levels in muscle and serum of broiler chickens fed diets reduced in
protein content. A total of 1,540 Cobb500 male chickens were distributed into 7 treatments, with 10 replicates with 22 birds each. The experimental diets were based on corn and soybean meal, and a control diet was formulated to satisfy broiler nutritional requirements. A basal diet with reduced
protein content was formulated to meet broiler nutritional requirements, except for
SID Arg levels. The experimental diets were obtained by adding
L-arginine to basal diets, meeting 6 different
SID Arg:Lys ratios (94, 100, 106, 112, 118, and 124%).
Body weight,
body weight gain, average daily feed intake, and feed conversion ratio were evaluated from 01 to 21 d old (trial 1) and from 22 to 44 d old (trial 2). At 21 and 44 d, in trials 1 and 2, respectively, birds were slaughtered to assess skin thickness (ST), skin strength (SS),
creatine level in muscle (CRM) and serum (CRS). Data were subjected to ANOVA, and treatments were compared to the control group by Dunnett's test (P ≤ 0.05). Regression analyses were performed to model the variables assessed and the ratios of
SID Arg:Lys. The
SID Arg:Lys ratios did not affect ADFI of broilers in both trials (P > 0.05), whereas it linearly increased the BW, BWG, and ST, in both trials (P < 0.001). The FCR of broilers linearly decreased, in trial 1 (P = 0.038) and trial 2 (P < 0.001). The CRM of birds had a linear effect (P < 0.001) in trial 1, and a quadratic effect (P = 0.001) in trial 2. The CRS and SS of broilers linearly increased, in trial 2 (P < 0.001). In conclusion, increasing
SID Arg:Lys ratios in diets reduced CP enhanced growth performance, skin quality and CR levels in muscle and serum of broiler chickens from 01 to 21 and 22 to 44 d old.