Background: B cells and
autoantibodies play an important role in the pathogenesis of
abdominal aortic aneurysm (AAA).
IgG glycosylations are highly valued as potential disease
biomarkers and therapeutic targets. Methods:
Lectin microarray was applied to analyze the expression profile of serum
IgG glycosylation in 75 patients with AAA, 68
autoimmune disease controls, and 100 healthy controls.
Lectin blots were performed to validate the differences. The clinical relevance of
lectins binding from the microarray results was explored in AAA patients. Results: Significantly lower binding level of SBA (preferred GalNAc) was observed for the AAA group compared with DCs (p < 0.001) and HCs (p = 0.049). A significantly lower binding level of ConA (preferred
mannose) was observed in patients with
aneurysm diameter >5 cm. Significantly higher binding of CSA (preferred GalNAc) was present for
dyslipidemia patients, whereas a lower binding level of AAL (preferred
fucose) was observed for hypertensive patients. Patients with diabetes had lower binding levels of IRA (preferred GalNAc) and HPA (preferred GalNAc) compared with those not with DM. PTL-L (R = 0.36, p = 0.0015, preferred GalNAc) was positively associated with
aneurysm diameters, whereas DSL (R = 0.28, p = 0.014, preferred (
GlcNAc)2-4) was positively associated with patients’ age. Symptomatic patients had a lower binding level of ConA (p = 0.032), and patients with
coronary heart disease had higher binding levels of STL (p = 0.0029, preferred GlcNAc). Patients with ILT bound less with black bean crude (p = 0.04, preferred GalNAc). Conclusions: AAA was associated with a decreased
IgG binding level of SBA (recognizing
glycan GalNAc). Symptomatic patients with
aneurysm <5 cm had a higher binding level of ConA (preferred
mannose).
Coronary heart disease and elder age were associated with increased
IgG bisecting GlcNAc.
IgG O-glycosylation (GalNAc) may play an important role in AAA pathogenesis and progression.