Abstract | BACKGROUND: OBJECTIVE: The aim of the present study was to explore the metabolic characteristics of patients with early-stage NAFLD based on the ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technology, to identify differential metabolites and perform functional analysis, and especially, to establish a novel early NAFLD clinical diagnostic tool. RESULTS: Compared with healthy controls, serum metabolite species increased significantly in early stage NAFLD patients. Expression of 88 metabolites including 1-naphthylmethanol, rosavin, and theophylline were up-regulated in early NAFLD, while 68 metabolites including 2-hydroxyphenylacetic acid and lysophosphatidylcholine (24:1(15Z)) were down-regulated. The early NAFLD classifier achieved a strong diagnostic efficiency in the discovery phases (80.99%) and was successfully verified in the validation phases (75.23%). CONCLUSIONS: These results advance our understanding about the composition and biological functions of serum metabolites of early NAFLD. In addition, serum metabolic markers can serve as an efficient diagnostic tool for the early-stage NAFLD.
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Authors | Xuemei Wang, Benchen Rao, Haiyu Wang, Chao Liu, Zhigang Ren, Zujiang Yu |
Journal | Bioscience reports
(Biosci Rep)
Vol. 42
Issue 10
(10 28 2022)
ISSN: 1573-4935 [Electronic] England |
PMID | 36124945
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 The Author(s). |
Chemical References |
- Lysophosphatidylcholines
- Theophylline
- Biomarkers
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Topics |
- Humans
- Non-alcoholic Fatty Liver Disease
- Chromatography, Liquid
- Lysophosphatidylcholines
- Theophylline
- Tandem Mass Spectrometry
- Metabolome
- Metabolomics
(methods)
- Biomarkers
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