Rational design of near-infrared ratiometric fluorescent probes for real-time tracking of β-galactosidase in vivo.

Abstract

β-Galactosidase (β-gal) is a hydrolytic enzyme in lysosomes and is also an important biomarker of cellular senescence and primary ovarian cancer. Therefore, real-time non-invasive detection of β-gal activity in vivo is of great significance for the prevention of cell senescence and early diagnosis of ovarian cancer. We designed an enzyme-activated proportional near-infrared (NIR) probe (Gal-Br-NO2) for real-time fluorescence quantification and capture of β-gal activity in vivo. The main characteristics of the Gal-Br-NO2 probe include short response time (less than 10 min), large Stokes displacement (155 nm), and near-infrared fluorescence emission (670 nm). The probe has also been successfully used to detect β-gal in ovarian cancer cells and senile cells and can accurately detect endogenous β-gal in zebrafish. Our work provides a potential tool for pre-clinical real-time tracking of β-gal activity in vivo and early diagnosis of disease.

• Authors: Shijun Chen, Mengyao Liu, Yunjiang Zi, Junyi He, Lin Wang, Yuanyuan Wu, Shicong Hou, Wenxue Wu
• Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Spectrochim Acta A Mol Biomol Spectrosc) Vol. 285 Pg. 121879 (Jan 15 2023) ISSN: 1873-3557 [Electronic] England
• PMID: 36122464 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier B.V. All rights reserved.

Chemical References

• Fluorescent Dyes
• Nitrogen Dioxide
• beta-Galactosidase

Topics

• Animals
• Female
• Humans
• Fluorescent Dyes
• Zebrafish
• Nitrogen Dioxide
• beta-Galactosidase
• Ovarian Neoplasms