Chemical profiling and identification of anti-osteoporosis chemical-markers of Cinnamomum cassia (L.) presl extracts using GC-MS and spectrum-activity analyses.

Abstract

Cinnamomum cassia (L.) Presl (cinnamon), an important folk medicine is widely used to prevent osteoporosis for long time in China. Our study aimed to investigate the anti-osteoporosis activity and mechanisms of cinnamon extracts obtained by supercritical CO2 extraction (SFE) and identify activity associated chemical components by gas chromatography-mass spectrometry. The cinnamon SFE exhibited superior anti-osteoporosis efficacy in an ovariectomised mice model to common alcohol extracts. It could induce calcified nodules and ALP activity, upregulate the mRNA expression of ALP, BMP-2, and RUNX2 in MC3T3-E1 cells. The major chemical classes of cinnamon extracts were alcohol esters (28.2%), and terpenes (16.1%). The spectrum-activity analysis indicated that the potential chemical-markers of extracts could be (E)-Cinnamaldehyde, γ-Sitosterol, and (Z, Z)-9,12-Octadecadienoic acid, which could induce the proliferation and ALP activity in MC3T3-E1 cells. Our study revealed the promising applications of the cinnamon SFE in prevention of osteoporosis, and identified its anti-osteoporosis associated compounds.

• Authors: Shan Zhao, Yali Wu, Yafang Qian, Yifan Qian, Shuai Xue, Junyan Chen, Qinghe Zeng, Mancang Gu
• Journal: Natural product research (Nat Prod Res) Vol. 37 Issue 11 Pg. 1902-1906 (Jun 2023) ISSN: 1478-6427 [Electronic] England
• PMID: 36098221 (Publication Type: Journal Article)

Chemical References

• Plant Extracts

Topics

• Animals
• Mice
• Cinnamomum aromaticum (chemistry, metabolism)
• Gas Chromatography-Mass Spectrometry
• Cinnamomum zeylanicum (chemistry)
• Medicine, Traditional
• Spectrum Analysis
• Plant Extracts (chemistry)