CCT196969 effectively inhibits growth and survival of melanoma brain metastasis cells.

Abstract

Melanomas frequently metastasize to the brain. Despite recent progress in the treatment of melanoma brain metastasis, therapy resistance and relapse of disease remain unsolved challenges. CCT196969 is a SRC family kinase (SFK) and Raf proto-oncogene, serine/threonine kinase (RAF) inhibitor with documented effects in primary melanoma cell lines in vitro and in vivo. Using in vitro cell line assays, we studied the effects of CCT196969 in multiple melanoma brain metastasis cell lines. The drug effectively inhibited proliferation, migration, and survival in all examined cell lines, with viability IC50 doses in the range of 0.18-2.6 μM. Western blot analysis showed decreased expression of p-ERK, p-MEK, p-STAT3 and STAT3 upon CCT196969 treatment. Furthermore, CCT196969 inhibited viability in two B-Raf Proto-Oncogene (BRAF) inhibitor resistant metastatic melanoma cell lines. Further in vivo studies should be performed to determine the treatment potential of CCT196969 in patients with treatment-naïve and resistant melanoma brain metastasis.

• Authors: Agathe Reigstad, Christina Frantzen Herdlevær, Emma Rigg, Tuyen Hoang, Ole Vidhammer Bjørnstad, Synnøve Nymark Aasen, Jasmin Preis, Claude Haan, Terje Sundstrøm, Frits Thorsen
• Journal: PloS one (PLoS One) Vol. 17 Issue 9 Pg. e0273711 ( 2022) ISSN: 1932-6203 [Electronic] United States
• PMID: 36084109 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 1-(3-tert-butyl-1-phenyl-1H-pyrazol-5-yl)-3-(2-fluoro-4-(3-oxo-3,4-dihydropyrido(3,2-b)pyrazin-8-yloxy)phenyl)urea
• Phenylurea Compounds
• Protein Kinase Inhibitors
• Pyrazines
• Proto-Oncogene Proteins B-raf

Topics

• Brain Neoplasms (drug therapy)
• Cell Line, Tumor
• Drug Resistance, Neoplasm
• Humans
• Melanoma (pathology)
• Mutation
• Neoplasm Recurrence, Local
• Phenylurea Compounds
• Protein Kinase Inhibitors (pharmacology)
• Proto-Oncogene Proteins B-raf (metabolism)
• Pyrazines