Role of Axl in target organ inflammation and damage due to hypertensive aortic remodeling.

Abstract

We have shown that excessive endothelial cell stretch causes release of growth arrest-specific 6 (GAS6), which activates the tyrosine kinase receptor Axl on monocytes and promotes immune activation and inflammation. We hypothesized that GAS6/Axl blockade would reduce renal and vascular inflammation and lessen renal dysfunction in the setting of chronic aortic remodeling. We characterized a model of aortic remodeling in mice following a 2-wk infusion of angiotensin II (ANG II). These mice had chronically increased pulse wave velocity, and their aortas demonstrated increased mural collagen. Mechanical testing revealed a marked loss of Windkessel function that persisted for 6 mo following ANG II infusion. Renal function studies showed a reduced ability to excrete a volume load, a progressive increase in albuminuria, and tubular damage as estimated by periodic acid Schiff staining. Treatment with the Axl inhibitor R428 beginning 2 mo after ANG II infusion had a minimal effect on aortic remodeling 2 mo later but reduced the infiltration of T cells, γ/δ T cells, and macrophages into the aorta and kidney and improved renal excretory capacity, reduced albuminuria, and reduced evidence of renal tubular damage. In humans, circulating Axl+/Siglec6+ dendritic cells and phospho-Axl+ cells correlated with pulse wave velocity and aortic compliance measured by transesophageal echo, confirming chronic activation of the GAS6/Axl pathway. We conclude that brief episodes of hypertension induce chronic aortic remodeling, which is associated with persistent low-grade inflammation of the aorta and kidneys and evidence of renal dysfunction. These events are mediated at least in part by GAS6/Axl signaling and are improved with Axl blockade.NEW & NOTEWORTHY In this study, a brief, 2-wk period of hypertension in mice led to progressive aortic remodeling, an increase in pulse wave velocity, and evidence of renal injury, dysfunction, and albuminuria. This end-organ damage was associated with persistent renal and aortic infiltration of CD8+ and γ/δ T cells. We show that this inflammatory response is likely due to GAS6/Axl signaling and can be ameliorated by blocking this pathway. We propose that the altered microvascular mechanical forces caused by increased pulse wave velocity enhance GAS6 release from the endothelium, which in turn activates Axl on myeloid cells, promoting the end-organ damage associated with aortic stiffening.

• Authors: Wei Chen, Justin P Van Beusecum, Liang Xiao, David M Patrick, Mingfang Ao, Shilin Zhao, Marcos G Lopez, Frederic T Billings 4th, Cristina Cavinato, Alexander W Caulk, Jay D Humphrey, David G Harrison
• Journal: American journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 323 Issue 5 Pg. H917-H933 (11 01 2022) ISSN: 1522-1539 [Electronic] United States
• PMID: 36083796 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Angiotensin II
• Collagen
• Intercellular Signaling Peptides and Proteins
• Periodic Acid
• Proto-Oncogene Proteins
• Receptor Protein-Tyrosine Kinases
• Axl Receptor Tyrosine Kinase

Topics

• Animals
• Humans
• Mice
• Albuminuria (prevention & control)
• Angiotensin II (pharmacology)
• Aorta (metabolism)
• Collagen
• Hypertension
• Inflammation (metabolism)
• Intercellular Signaling Peptides and Proteins
• Kidney Diseases
• Periodic Acid
• Proto-Oncogene Proteins (metabolism)
• Pulse Wave Analysis
• Receptor Protein-Tyrosine Kinases (metabolism)
• Axl Receptor Tyrosine Kinase