Ginsenoside Rd inhibits migration and invasion of tongue cancer cells through H19/miR-675-5p/CDH1 axis.

Abstract

OBJECTIVE:

Tongue squamous cell carcinoma (TSCC) is an oral cancer, with high malignancy and frequent early migration and invasion. Only a few drugs can treat tongue cancer. Ginsenoside Rd is a ginseng extract with anti-cancer effects. Many noncoding RNAs are abnormally expressed in tongue cancer, thus influencing its occurrence and development. H19 and miR-675-5p can promote cancer cell growth. This study aimed to analyze the regulation effect of ginsenoside Rd on H19 and miR-675-5p in tongue cancer.

METHODOLOGY:

We used CCK8 and flow cytometry to study the growth and apoptosis. Transwell assay was used to assess invasion; wound-healing assay to assess migration; and colony formation assays to test the ability of cells to form colonies. H19, miR-675-5p, and CDH1 expressions were analyzed by qPCR. E-cadherin expression was detected using western blot. CRISPR/cas9 system was used for CDH1 knockout.

RESULTS:

Ginsenoside Rd inhibited the growth and increased the apoptosis of SCC9 cells. Ginsenoside Rd also inhibited the migration and invasion of SCC9 cells. H19 and miR-675-5p were highly expressed, while CDH1 and E-cadherin expressions were low. H19 and miR-675-5p promoted SCC9 metastasis. In contrast, CDH1 and E-cadherin inhibited the metastasis of SCC9 cells. Bioinformatics analysis showed that miR-675-5p was associated with CDH1. H19 and miR-675-5p expressions decreased after ginsenoside Rd treatment, while CDH1 and E-cadherin expressions increased.

CONCLUSIONS:

Ginsenoside Rd inhibits tongue cancer cell migration and invasion via the H19/miR-675-5p/CDH1 axis.

• Authors: Lu Chang, Dongxu Wang, Shaoning Kan, Ming Hao, Huimin Liu, Zhijing Yang, Qianyun Xia, Weiwei Liu
• Journal: Journal of applied oral science : revista FOB (J Appl Oral Sci) Vol. 30 Pg. e20220144 ( 2022) ISSN: 1678-7765 [Electronic] Brazil
• PMID: 36074434 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• CDH1 protein, human
• Cadherins
• Ginsenosides
• Histones
• MIRN675 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• ginsenoside Rd

Topics

• Antigens, CD (pharmacology)
• Cadherins
• Carcinoma, Squamous Cell
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Ginsenosides
• Histones (metabolism)
• Humans
• MicroRNAs (genetics)
• RNA, Long Noncoding (genetics, metabolism, pharmacology)
• Tongue (metabolism)
• Tongue Neoplasms (drug therapy)