As a major complication after
percutaneous coronary intervention (PCI) in patients who suffer from
coronary artery disease, in-
stent restenosis (ISR) poses a significant challenge for clinical management. A
miRNA-
mRNA regulatory network of ISR can be constructed to better reveal the occurrence of ISR. The relevant data set from the Gene Expression Omnibus (GEO) database was downloaded, and 284 differentially expressed
miRNAs (DE-
miRNAs) and 849 differentially expressed mRNAs (DE-mRNAs) were identified. As predicted by online tools, 65 final functional genes (FmRNAs) were overlapping DE-mRNAs and DE-
miRNAs target genes. In the biological process (BP) terms of gene ontology (GO) functional analysis, the FmRNAs were mainly enriched in the cellular response to
peptide, epithelial cell proliferation, and response to
peptide hormone. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, the FmRNAs were mainly enriched in
breast cancer, endocrine resistance, and
Cushing syndrome. Jun proto-oncogene,
activator protein-1 (AP-1)
transcription factor subunit (JUN),
insulin-like growth factor 1 receptor (IGF1R), member RAS oncogene family (RAB14), specificity
protein 1 (SP1),
protein tyrosine phosphatase nonreceptor type 1 (PTPN1), DDB1 and CUL4 associated
factor 10 (DCAF10),
retinoblastoma-
binding protein 5 (RBBP5), and eukaryotic
initiation factor 4A-I (EIF4A1) were hub genes in the protein-protein interaction network (PPI network). The
miRNA-
mRNA network containing DE-
miRNAs and hub genes was built. Hsa-miR-139-5p-JUN, hsa-miR-324-5p-SP1 axis pairs were found in the
miRNA-
mRNA network, which could promote ISR development. The aforementioned results indicate that the
miRNA-
mRNA network constructed in ISR has a regulatory role in the development of ISR and may provide new approaches for clinical treatment and experimental development.