Abstract | BACKGROUND: METHODS: A chronic migraine model was established by repeated inflammatory soup stimulation. Mechanical, periorbital, and thermal pain thresholds were assessed by the application of von Frey filaments and radiant heat. The mRNA and protein expression levels of the dopamine D2 receptor were analyzed by qRT‒PCR and western blotting. Colocalization of the dopamine D2 receptor and the GLUA1-containing AMPAR was observed by immunofluorescence. A dopamine D2 receptor agonist ( quinpirole) and antagonist ( sulpiride), a PI3K inhibitor ( LY294002), a PI3K pathway agonist (740YP), and a GLUA1-containing AMPAR antagonist ( NASPM) were administered to confirm the effects of the dopamine D2 receptor, the PI3K pathway and GULA1 on central sensitization and the GLUA1-containing AMPAR trafficking. Transmission electron microscopy and Golgi-Cox staining were applied to assess the impact of the dopamine D2 receptor and PI3K pathway on synaptic morphology. Fluo-4-AM was used to clarify the role of the dopamine D2 receptor and PI3K signaling on neuronal calcium influx. The Src family kinase (SFK) inhibitor PP2 was used to explore the effect of Src kinase on GLUA1-containing AMPAR trafficking and the PI3K signaling pathway. RESULTS: CONCLUSION:
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Authors | Wei Zhang, Ming Lei, Qianwen Wen, Dunke Zhang, Guangcheng Qin, Jiying Zhou, Lixue Chen |
Journal | The journal of headache and pain
(J Headache Pain)
Vol. 23
Issue 1
Pg. 98
(Aug 10 2022)
ISSN: 1129-2377 [Electronic] England |
PMID | 35948867
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Receptors, AMPA
- Receptors, Dopamine D2
- Quinpirole
- Sulpiride
- Calcium
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Topics |
- Animals
- Calcium
(metabolism)
- Central Nervous System Sensitization
(physiology)
- Male
- Migraine Disorders
(metabolism)
- Pain
- Phosphatidylinositol 3-Kinases
(metabolism)
- Quinpirole
(pharmacology)
- Rats
- Receptors, AMPA
(metabolism)
- Receptors, Dopamine D2
(metabolism)
- Signal Transduction
- Sulpiride
(pharmacology)
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