Abstract |
Quantitative phase imaging (QPI) measures the growth rate of individual cells by quantifying changes in mass versus time. Here, we use the breast cancer cell lines MCF-7, BT-474, and MDA-MB-231 to validate QPI as a multiparametric approach for determining response to single-agent therapies. Our method allows for rapid determination of drug sensitivity, cytotoxicity, heterogeneity, and time of response for up to 100,000 individual cells or small clusters in a single experiment. We find that QPI EC50 values are concordant with CellTiter-Glo (CTG), a gold standard metabolic endpoint assay. In addition, we apply multiparametric QPI to characterize cytostatic/cytotoxic and rapid/slow responses and track the emergence of resistant subpopulations. Thus, QPI reveals dynamic changes in response heterogeneity in addition to average population responses, a key advantage over endpoint viability or metabolic assays. Overall, multiparametric QPI reveals a rich picture of cell growth by capturing the dynamics of single-cell responses to candidate therapies.
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Authors | Edward R Polanco, Tarek E Moustafa, Andrew Butterfield, Sandra D Scherer, Emilio Cortes-Sanchez, Tyler Bodily, Benjamin T Spike, Bryan E Welm, Philip S Bernard, Thomas A Zangle |
Journal | Communications biology
(Commun Biol)
Vol. 5
Issue 1
Pg. 794
(08 08 2022)
ISSN: 2399-3642 [Electronic] England |
PMID | 35941353
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2022. The Author(s). |
Chemical References |
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Topics |
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Breast Neoplasms
(drug therapy, metabolism)
- Cell Proliferation
- Drug Evaluation, Preclinical
- Early Detection of Cancer
- Female
- Humans
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