Human Umbilical Cord Wharton Jelly Cells Treatment Prevents Osteoporosis Induced by D-Galactose.

Abstract

Methods:

Sixteen male mice were randomly divided into 4 groups: control (ordinary feeding), D-gal (D-galactose) group, D-gal + MSC (human umbilical cord Wharton jelly cells), and D-gal + MSC-TNFα groups. Except for the control group (fed with same amount of saline solution), other mice received gastric feeding of 250 mg/kg D-galactose every day for 8 weeks. TNFα (10 ng/mL for 24 h) cocultured or noncocultured HUCWJCs (5 × 105) were suspended in 0.1 ml of sterile PBS and injected into tail veins every other week in D-gal + MSC-TNFα and D-gal + MSC groups, respectively, and only 0.1 ml of sterile PBS for control and D-gal groups. The bone mass was detected by qPCR, ELISA, microcomputed tomography (μCT), and hematoxylin-eosin staining. Proliferation, apoptosis, and differentiation of periosteal-derived osteoblasts (POB) were assessed. Transwell assay and scratch healing were performed to detect POB migration and invasion ability. The effect of HUCWJCs on POB signaling pathway expression was evaluated by immunoblotting.

Results:

The malondialdehyde (MDA) in serum was higher and superoxide dismutase (SOD) was lower in the D-gal group compared to the other groups (p < 0.05). Mice in D-gal group showed significantly decreased bone mass when compared to the control group, while HUCWJCs treatment partially rescued the phenotype, as demonstrated by μCT and histology (p < 0.05). Mechanically, HUCWJCs treatment partially promoted proliferation and migration and decreased apoptosis of POB induced by oxidative stress via activating the mitogen-activated protein kinase (MAPK) signaling pathway.

Conclusion:

HUCWJCs can prevent the progression of osteoporosis by inhibiting oxidative stress, which may act by regulating osteoblasts fate through the MAPK signaling pathway.

• Authors: Qiang Wang, Zhiqiang Gao, Kai Guo, Jiawei Lu, Feng Wang, Tongde Wu, Yufeng Huang, Desheng Wu
• Journal: International journal of clinical practice (Int J Clin Pract) Vol. 2022 Pg. 4593443 ( 2022) ISSN: 1742-1241 [Electronic] India
• PMID: 35936064 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Qiang Wang et al.

Chemical References

• Tumor Necrosis Factor-alpha
• Galactose

Topics

• Animals
• Galactose (metabolism, pharmacology)
• Humans
• Male
• Mesenchymal Stem Cells (metabolism)
• Mice
• Osteoporosis
• Oxidative Stress
• Tumor Necrosis Factor-alpha (metabolism, pharmacology)
• Umbilical Cord (metabolism)
• X-Ray Microtomography