Aim
Triple negative breast cancer cells (TNBC) are the population of
breast cancer cells that are responsible for
cancer recurrence and apoptosis resistance. Unfortunately, current
therapies have limited efficacy to TNBC population due to apoptosis resistance and chemoresistance. Tumour suppressor p53 and
survivin are primary targets for TNBC
therapy. Consequently, a search for a natural compound which targets p53 and
survivin is needed to further advance TNBC treatment.
Curcuma longa extract (CL), a natural compound induces apoptosis in several
cancer cells by targeting various molecules and possess fewer side effects. However, a possible potential of CL as p53- and
survivin modulating agent in TNBC cells has not been investigated. Methods MDAMB-231 cells were treated with several concentration of CL, after which, viability, p53 gene expression, surviving
protein expression, and
caspase-3 protein expression were evaluated. Results After 24-h treatment, CL possessed cytotoxic effect with IC50 value of 13 μg/mL. Treatment with 1.625, 3.25, 6.5, and 13 μg/mL of CL resulted in 2.70-25.80% increase in
caspase-3 expression levels followed by 94.60 - 21.60% decrease in
survivin protein levels. CL induced remarkably p53 gene expression ratio up to 5-fold at 13 μg/mL.
Survivin protein levels were inversely proportional to p53 accumulation levels. Low
survivin protein levels combined with high levels of p53 accumulation were correlated to higher apoptotic rates. Conclusion p53 and
survivin as molecular targets of CL contribute to caspase-3-dependent apoptosis in TNBC cells and this compound represents an attractive p53- and
survivin modulating agent in TNBC.