Generation and Culture of Organotypic Breast Carcinoma Spheroids for the Study of Drug Response in a 3D Microfluidic Device.

Abstract

Breast cancer (BC) is a leading cause of cancer death among women worldwide. To better understand and predict therapeutic response in BC patient developing a fast, low-cost, and reliable preclinical tumor from patient's tumor specimen is needed. Here, we describe the development of a preclinical model of BC through the generation and ex vivo culture of patient-derived organotypic tumor spheroids (PDOTS) in a 3D microfluidic device. Moreover, the real-time screening of conventional chemotherapy agents on cultured PDOTS is also described.

• Authors: Hamidreza Aboulkheyr Es, Amir Reza Aref, Majid Ebrahimi Warkiani
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2535 Pg. 49-57 ( 2022) ISSN: 1940-6029 [Electronic] United States
• PMID: 35867221 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Topics

• Breast Neoplasms (drug therapy)
• Cell Culture Techniques
• Female
• Humans
• Lab-On-A-Chip Devices
• Microfluidic Analytical Techniques
• Spheroids, Cellular