RASSF8-AS1 displays low expression in colorectal cancer and up-regulates RASSF8 to suppress cell invasion and migration.

Abstract

BACKGROUND:

Colorectal cancer (CRC) is among the most prevalent cancers. Long non-coding RNAs (lncRNAs) are important participant in various cancers. Based on the literature, lncRNA RASSF8-AS1 inhibits laryngeal squamous cell carcinoma (LSCC) malignant progression. However, the role of RASSF8-AS1 in CRC remains unclear.

PURPOSE:

This study centered on uncovering the role of RASSF8-AS1 and its related regulatory mechanisms in CRC cells.

METHODS:

RT-qPCR and western blot were performed to examine the expression of target genes. Functional assays were conducted to determine the effect of target genes on the migration and invasion of CRC cells. Mechanism assays were also carried out to figure out the specific downstream mechanisms of RASSF8-AS1. In vivo assays were also involved.

RESULTS:

The expression of RASSF8-AS1 and RASSF8 was positively correlated in CRC, and the two genes were down-regulated in CRC cells and tissues. Moreover, CRC cell invasion and migration as well as xenograft CRC tumor growth suppressed by RASSF8-AS1 overexpression were entirely recovered by RASSF8 knockdown or partially rescued by miR-33a-5p augment. As for the downstream mechanism, RASSF8-AS1 sponged miR-33a-5p to up-regulate RASSF8, or recruited HNRNPC to stabilize RASSF8 mRNA.

CONCLUSION:

RASSF8-AS1 modulates miR-33a-5p/HNRNPC/RASSF8 axis to further impede CRC cell invasion and migration.

AVAILABILITY OF DATA:

The research data is confidential.

• Authors: Xi Zhang, Pengjin Sun, Wei Yang, Sizhu Li, Heng Jia, Wengang Wang, Shuai Jiang, Lei Wang, Xianglin Liu, Liang Liang, Wei Zi
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 237 Pg. 153996 (Sep 2022) ISSN: 1618-0631 [Electronic] Germany
• PMID: 35839610 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier GmbH. All rights reserved.

Chemical References

• MicroRNAs
• RASSF8 protein, human
• RNA, Long Noncoding
• RNA, Messenger
• Tumor Suppressor Proteins

Topics

• Humans
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Colorectal Neoplasms (pathology)
• Gene Expression Regulation, Neoplastic (genetics)
• MicroRNAs (genetics, metabolism)
• RNA, Long Noncoding (genetics, metabolism)
• RNA, Messenger
• Tumor Suppressor Proteins (metabolism)
• Animals