Abstract |
Enrofloxacin (ENR) is a fluoroquinolone antibiotic that has been used to treat bacterial diseases in aquaculture extensively. ENR might accumulate in the body and cause severe liver damage. Therefore, it is necessary to develop a method for rapid detection of ENR. Herein, amine monomers with different numbers of amino groups, charge and length (1,3- diaminoguanidine monohydrochloride: DMGH, triaminoguanidine hydrochloride: TAGH, hydrazine: H) were used to regulate the fluorescence of benzotrithiophene tricar-baldehyde (BTT)-based covalent organic frameworks (COFs) for real-time visual onsite assays of ENR for the first time. The C3 symmetric COFBTT-TAGH only had one emission peak at 540 nm, while C6 symmetric COFBTT-DMGH and COFBTT-H had two emission peaks. COFBTT-TAGH could achieve the color transition from green to blue with increasing ENR. Thus test paper and gel were designed as real-time detection tools, combined with a smartphone APP. The detection limits obtained by RGB analysis were 106.2 nM for test paper and 26.00 nM for test gel, respectively. This method was applied to detect ENR in fish and clam metabolite successfully. This work also provides important reference to regulate fluorescence of COFs according to actual application.
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Authors | Li Yang, Mengyao Li, Lijuan Kuang, Yiheng Li, Lili Chen, Chunhua Lin, Li Wang, Yonghai Song |
Journal | Biosensors & bioelectronics
(Biosens Bioelectron)
Vol. 214
Pg. 114527
(Oct 15 2022)
ISSN: 1873-4235 [Electronic] England |
PMID | 35797935
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Anti-Bacterial Agents
- Fluoroquinolones
- Metal-Organic Frameworks
- Enrofloxacin
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Topics |
- Animals
- Anti-Bacterial Agents
(analysis)
- Aquaculture
- Biosensing Techniques
- Enrofloxacin
(analysis)
- Fluoroquinolones
(analysis)
- Metal-Organic Frameworks
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