EIF5A2 promotes proliferation and invasion of intrahepatic cholangiocarcinoma cells.

Abstract

PURPOSE:

Intrahepatic cholangiocarcinoma (ICC) can invade and metastasize. EIF5A2 is involved in the invasive metastatic process of several digestive malignancies. However, its role in ICC is yet to be elucidated.

METHODS:

Immunohistochemistry (IHC) and Western blot (WB) were used to detect the level of EIF5A2 in the tumor specimens of ICC patients and evaluate the correlation between its expression and clinicopathological characteristics. The significance of EIF5A2 in the prognosis of ICC patients was further evaluated by Kaplan-Meier and Cox regression analysis. In addition, CCK-8, EdU, Transwell invasion, and scratch assays were utilized to detect tumor cell proliferation, invasion, and metastasis. Furthermore, the role of EIF5A2 in ICC cells was evaluated after modification of EIF5A2 expression.

RESULTS:

The level of EIF5A2 protein was significantly higher in ICC than in adjacent tissues. This high expression in the tumor samples was significantly associated with malignant phenotypes, such as lymph node metastasis (LNM), microvascular or bile duct invasion, and poor differentiation. ICC patients with high expression of EIF5A2 had short overall survival and a high cumulative recurrence rate. The multifactorial analysis showed that EIF5A2 is an independent prognostic marker. Furthermore, high levels of EIF5A2 may activate the PI3K/AKT/mTOR signaling pathway and upregulate Cyclin D1, Cyclin D3, MMP2, and MMP9 to promote ICC cell proliferation, migration, and invasion.

CONCLUSION:

The current study found that EIF5A2 promotes ICC progression and is a prognostic biomarker and candidate therapeutic target for ICC patients.

• Authors: Shao-Hua Yang, Sheng Hu, Qiang Kang, Li-Xin Liu, Qun Wei, Zhi-Mei Song, Yi-Hui Chen, Zhou-Jun Liao, Yun-Feng Huan, Xue-Jun Wang, Hao Zou, Xiao-Wen Zhang
• Journal: Clinics and research in hepatology and gastroenterology (Clin Res Hepatol Gastroenterol) 2022 Aug-Sep Vol. 46 Issue 7 Pg. 101991 ISSN: 2210-741X [Electronic] France
• PMID: 35792239 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Peptide Initiation Factors
• RNA-Binding Proteins

Topics

• Bile Duct Neoplasms
• Bile Ducts, Intrahepatic (pathology)
• Cell Proliferation (physiology)
• Cholangiocarcinoma
• Humans
• Peptide Initiation Factors
• Phosphatidylinositol 3-Kinases (metabolism)
• Prognosis
• RNA-Binding Proteins
• Eukaryotic Translation Initiation Factor 5A