Chl1, an ATP-Dependent DNA Helicase, Inhibits DNA:RNA Hybrids Formation at DSB Sites to Maintain Genome Stability in S. pombe.

Abstract

As an ATP-dependent DNA helicase, human ChlR1/DDX11 (Chl1 in yeast) can unwind both DNA:RNA and DNA:DNA substrates in vitro. Studies have demonstrated that ChlR1 plays a vital role in preserving genome stability by participating in DNA repair and sister chromatid cohesion, whereas the ways in which the biochemical features of ChlR1 function in DNA metabolism are not well understood. Here, we illustrate that Chl1 localizes to double-strand DNA break (DSB) sites and restrains DNA:RNA hybrid accumulation at these loci. Mutation of Chl1 strongly impairs DSB repair capacity by homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways, and deleting RNase H further reduces DNA repair efficiency, which indicates that the enzymatic activities of Chl1 are needed in Schizosaccharomyces pombe. In addition, we found that the Rpc37 subunit of RNA polymerase III (RNA Pol III) interacts directly with Chl1 and that deletion of Chl1 has no influence on the localization of Rpc37 at DSB site, implying the role of Rpc37 in the recruitment of Chl1 to this site.

• Authors: Deyun He, Zhen Du, Huiling Xu, Xiaoming Bao
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 12 (Jun 14 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 35743069 (Publication Type: Journal Article)

Chemical References

• CHL1 protein, human
• Cell Adhesion Molecules
• RNA
• DNA
• DNA Helicases
• DDX11 protein, human
• DEAD-box RNA Helicases

Topics

• Cell Adhesion Molecules (metabolism)
• DEAD-box RNA Helicases (genetics, metabolism)
• DNA (metabolism)
• DNA Breaks, Double-Stranded
• DNA End-Joining Repair
• DNA Helicases (genetics, metabolism)
• DNA Repair
• Genomic Instability
• Humans
• RNA (metabolism)
• Saccharomyces cerevisiae (metabolism)
• Schizosaccharomyces (metabolism)