Honey peach (Prunus persica L.) is highly nutritious; it is an excellent source of
sugars,
proteins,
amino acids,
vitamins, and
mineral elements. However, it is a perishable climacteric fruit that is difficult to preserve. In this study, "Feicheng" honey peach fruit was used as a test material to investigate the synergistic preservation effect of
1-methylcyclopropene (1-MCP) and
laser microporous film (LMF). The peach fruits were fumigated for 24 h with 2 μL L-1
1-MCP, then packed in LMF. In comparison with the control treatment,
1-MCP + LMF treatment markedly decreased the respiration rate,
weight loss, and rot rate of peach fruits. Moreover, the combination of
1-MCP and LMF suppressed the increase in soluble solids (SS) and reducing
sugars (RS), as well as the decrease in titratable
acid (TA) and
ascorbic acid (AsA). The combined application also maintained a high protopectin content and low soluble
pectin content; it reduced the accumulation of
superoxide anions (O2-) and
hydrogen peroxide (H2O2). Except in a few samples, the
catalase (CAT) and
ascorbate peroxidase (APX) activities were higher when treated by
1-MCP + LMF. Conversely, the
phenylalanine deaminase (PAL),
peroxidase (POD),
lipase,
lipoxygenase (LOX),
polygalacturonase (PG), β-
glucosidase, and
cellulase (Cx) activities were lower than in the control. Furthermore,
1-MCP + LMF treatment reduced the relative abundances of dominant pathogenic fungi (e.g., Streptomyces, Stachybotrys, and Issa sp.). The combined treatment improved the relative abundances of antagonistic fungi (e.g., Aureobasidium and Holtermanniella). The results indicated that the co-application of
1-MCP and LMF markedly reduced
weight loss and spoilage, delayed the decline of nutritional quality, and inhibited the physiological and biochemical metabolic activities of peach during storage. These changes extended its shelf-life to 28 days at 5 °C. The results provide a reference for the commercial application of this technology.