Intervertebral disc degeneration (IVDD) is a complex process involving many factors, among which excessive senescence of nucleus pulposus cells is considered to be the main factor. Our previous study found that
metformin can inhibit senescence in nucleus pulposus cells; however, the mechanism of such an action was still largely unknown. In the current study, we found that
metformin inactivates the cGAS-
STING pathway during oxidative stress. Furthermore, knockdown of
STING (also known as STING1) suppresses senescence, indicating that
metformin might exert its effect through the cGAS-
STING pathway. Damaged
DNA is a major inducer of the activation of the cGAS-
STING pathway. Mechanistically, our study showed that DNA damage was reduced during
metformin treatment; however, suppression of autophagy by
3-methyladenine (3-MA) treatment compromised the effect of
metformin on DNA damage. In vivo studies also showed that 3-MA might diminish the
therapeutic effect of
metformin on IVDD. Taken together, our results reveal that
metformin may suppress senescence via inactivating the cGAS-
STING pathway through autophagy, implying a new application for
metformin in cGAS-
STING pathway-related diseases.