N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity.

Abstract

Phosphomannomutase 2 (PMM2) deficiency is the most prevalent congenital disorder of glycosylation. It is associated with coagulopathy, including protein C deficiency. Since all components of the anticoagulant and cytoprotective protein C system are glycosylated, we sought to investigate the impact of an N-glycosylation deficiency on this system as a whole. To this end, we developed a PMM2 knockdown model in the brain endothelial cell line hCMEC/D3. The resulting PMM2low cells were less able to generate activated protein C (APC), due to lower surface expression of thrombomodulin and endothelial protein C receptor. The low protein levels were due to downregulated transcription of the corresponding genes (THBD and PROCR, respectively), which itself was related to downregulation of transcription regulators Krüppel-like factors 2 and 4 and forkhead box C2. PMM2 knockdown was also associated with impaired integrity of the endothelial cell monolayer-partly due to an alteration in the structure of VE-cadherin in adherens junctions. The expression of protease-activated receptor 1 (involved in the cytoprotective effects of APC on the endothelium) was not affected by PMM2 knockdown. Thrombin stimulation induced hyperpermeability in PMM2low cells. However, pretreatment of cells with APC before thrombin simulation was still associated with a barrier-protecting effect. Taken as a whole, our results show that the partial loss of PMM2 in hCMEC/D3 cells is associated with impaired activation of protein C and a relative increase in barrier permeability.

• Authors: Tiffany Pascreau, François Saller, Elsa P Bianchini, Dominique Lasne, Arnaud Bruneel, Christelle Reperant, François Foulquier, Cécile V Denis, Pascale De Lonlay, Delphine Borgel
• Journal: Thrombosis and haemostasis (Thromb Haemost) Vol. 122 Issue 9 Pg. 1469-1478 (Sep 2022) ISSN: 2567-689X [Electronic] Germany
• PMID: 35717947 (Publication Type: Journal Article)
• Copyright: Thieme. All rights reserved.

Chemical References

• Protein C
• Thrombin
• Phosphotransferases (Phosphomutases)

Topics

• Congenital Disorders of Glycosylation
• Endothelium
• Glycosylation
• Humans
• Phosphotransferases (Phosphomutases) (deficiency)
• Protein C
• Thrombin