Protein oxidation marker, α-amino adipic acid, impairs proteome of differentiated human enterocytes: Underlying toxicological mechanisms.

Abstract

Protein oxidation and oxidative stress are involved in a variety of health disorders such as colorectal adenomas, inflammatory bowel's disease, neurological disorders and aging, among others. In particular, the specific final oxidation product from lysine, the α-amino adipic acid (α-AA), has been found in processed meat products and emphasized as a reliable marker of type II diabetes and obesity. Currently, the underlying mechanisms of the biological impairments caused by α-AA are unknown. To elucidate the molecular basis of the toxicological effect of α-AA, differentiated human enterocytes were exposed to dietary concentrations of α-AA (200 μM) and analyzed by flow cytometry, protein oxidation and proteomics using a Nanoliquid Chromatography-Orbitrap MS/MS. Cell viability was significantly affected by α-AA (p < 0.05). The proteomic study revealed that α-AA was able to alter cell homeostasis through impairment of the Na+/K+-ATPase pump, energetic metabolism, and antioxidant response, among other biological processes. These results show the importance of dietary oxidized amino acids in intestinal cell physiology and open the door to further studies to reveal the impact of protein oxidation products in pathological conditions.

• Authors: S Díaz-Velasco, J Delgado, F J Peña, Mario Estévez
• Journal: Biochimica et biophysica acta. Proteins and proteomics (Biochim Biophys Acta Proteins Proteom) Vol. 1870 Issue 7 Pg. 140797 (07 01 2022) ISSN: 1878-1454 [Electronic] Netherlands
• PMID: 35691541 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers
• Proteome

Topics

• Biomarkers
• Diabetes Mellitus, Type 2 (metabolism)
• Enterocytes (metabolism)
• Humans
• Proteome (metabolism)
• Proteomics
• Tandem Mass Spectrometry