A combined experimental and computational study to discover novel tyrosinase inhibitors.

Abstract

Depigmenting properties of tyrosinase inhibitors (TAi) boosted the search for new compounds applicable in cosmetics. Kojic acid, a 3-hydroxy-4-pyrone, is the most studied tyrosinase inhibitor but undesirable side effects, like dermatitis, and unspecified mechanism led to its exclusion in several countries. To discover safer and more efficient TA, we evaluated tyrosinase inhibitory effect of twelve 3-hydroxy-4-pyridinones (3,4-HPO) in vitro and considering the two reaction steps of inhibition in mushroom tyrosinase enzyme. In parallel we performed molecular docking studies in human and mushroom enzymes. Ligands I6 and I11 were the most effective compounds considering their inhibitory activity in both reaction steps. Our studies revealed that I6 has a non-competitive and mixed type of inhibition for monophenolase and diphenolase activity, while ligand I11 showed a mixed and competitive inhibition type for each reaction step. Molecular Docking results indicated that ligands tend to bind the enzyme by coordinating directly with the binuclear cooper centre and highlighted the relevance of voluminous and non-polar substituents at R2 to avoid the binding of the ligands to the enzyme. The work clarifies the type of inhibition established for kojic acid and points out the differences found for the set of 3,4-HPO chelators studied as prospective tyrosinase inhibitors.

• Authors: Luísa M P F Amaral, Tânia Moniz, Andreia Leite, Ana Oliveira, Pedro Fernandes, Maria João Ramos, Alberto N Araújo, Marisa Freitas, Eduarda Fernandes, Maria Rangel
• Journal: Journal of inorganic biochemistry (J Inorg Biochem) Vol. 234 Pg. 111879 (09 2022) ISSN: 1873-3344 [Electronic] United States
• PMID: 35679744 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022. Published by Elsevier Inc.

Chemical References

• Enzyme Inhibitors
• Monophenol Monooxygenase

Topics

• Agaricales (enzymology)
• Enzyme Inhibitors (chemistry, pharmacology)
• Humans
• Molecular Docking Simulation
• Monophenol Monooxygenase (antagonists & inhibitors, chemistry)
• Prospective Studies