Protein corona-coated immunomagnetic nanoparticles with enhanced isolation of circulating tumor cells.

Abstract

Immunomagnetic nanoparticles (IMNs) have been widely developed as a detection tool to isolate rare circulating tumor cells (CTCs) from whole blood as a potential method for early cancer diagnosis, metastasis examination, and treatment guidance. However, a spontaneous interaction between nanoparticles and proteins results in the formation of a protein corona that reduces the performance of IMNs when they enter body fluids. To address this issue, the protein corona was precoated onto magnetic nanoparticles (C-MNs), and then their surfaces were conjugated with an immuno-antibody. The adsorption of proteins on C-MNs was decreased 6-fold and non-specific cell binding was reduced 5-fold, compared with magnetic nanoparticles (MNs). Furthermore, the immuno-antibody functionalized C-MNs (IC-MNs) maintained highly specific CTC capture performance when exposed to blood plasma. By using artificial spiked blood samples, IC-MNs exhibited 90.2% CTC isolation efficiency, compared with 60.3% by using IMNs. IC-MNs also successfully captured CTCs with high purity in 24 out of 26 female breast cancer patient blood samples. This work demonstrated that a novel preformed protein corona strategy can provide a useful clinically applicable diagnostic tool.

• Authors: Xinbang Jiang, Xiangyun Zhang, Chen Guo, Yameng Yu, Boya Ma, Zhuang Liu, Yamin Chai, Lichun Wang, Yunzheng Du, Biao Wang, Nan Li, Dong Dong, Yueguo Li, Xinglu Huang, Lailiang Ou
• Journal: Nanoscale (Nanoscale) Vol. 14 Issue 23 Pg. 8474-8483 (Jun 16 2022) ISSN: 2040-3372 [Electronic] England
• PMID: 35661186 (Publication Type: Journal Article)

Chemical References

• Protein Corona

Topics

• Breast Neoplasms (diagnosis, pathology)
• Cell Line, Tumor
• Cell Separation
• Female
• Humans
• Immunomagnetic Separation (methods)
• Nanoparticles
• Neoplastic Cells, Circulating (metabolism)
• Protein Corona