Isoorientin ameliorates OVA-induced asthma in a murine model of asthma.

Abstract

Allergic asthma which is induced by ovalbumin (OVA) is a chronic airway inflammation disease. Isoorientin (Iso) is a natural C-glucosyl flavone with many biological properties. We aimed to evaluate the effectiveness of Iso on OVA-induced allergic asthma. A total of 30 C57BL/6 mice were randomly divided into five groups: control group, OVA group, Dex (dexamethasone, 10 mg/kg) group, low-dose Iso group (Iso-L, 25 mg/kg), and high-dose Iso group (Iso-H, 50 mg/kg). The serum and bronchoalveolar lavage fluid (BALF) were collected for biochemical parameters, the lung tissue was collected for hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC), and western blot. The levels of IL-4, IL-5, IL-13, malondialdehyde (MDA), NO, and reactive oxygen species (ROS) in Iso-L and Iso-H groups were significantly lower than that in model group (p < 0.05). Simultaneously, the levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity were higher than that in model group (p < 0.05). Iso significantly ameliorated airway hyperresponsiveness. Meanwhile, H&E staining revealed that mice treated with Iso resulted in the ameliorated inflammatory cell infiltration and a reduction in interstitial thickening. The nuclear factor erythroid 2-like 2 (Nrf2) and HO-1 protein expression in Iso-L and Iso-H groups were enhanced over that in model group, while p-NF-κB-p65 and p-IκB-α protein expression was decreased (p < 0.05). Our research indicated that Iso alleviated the OVA-induced allergic asthma, and this effect can be explained by the modulation of Nrf2/HO-1 and NF-κB signaling pathway; thus, the results providing a therapeutic rationale for the treatment of Iso on allergic asthma.

• Authors: Shuai Liang, Yuanyuan Zhao, Guozhen Chen, Chunxiao Wang
• Journal: Experimental biology and medicine (Maywood, N.J.) (Exp Biol Med (Maywood)) Vol. 247 Issue 16 Pg. 1479-1488 (08 2022) ISSN: 1535-3699 [Electronic] England
• PMID: 35658632 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Interleukin-13
• Interleukin-5
• NF-E2-Related Factor 2
• NF-kappa B
• Reactive Oxygen Species
• NF-KappaB Inhibitor alpha
• Interleukin-4
• Malondialdehyde
• Dexamethasone
• Ovalbumin
• homoorientin
• Glutathione Peroxidase
• Superoxide Dismutase
• Luteolin
• Eosine Yellowish-(YS)
• Hematoxylin

Topics

• Animals
• Asthma (chemically induced, drug therapy, metabolism)
• Cytokines (metabolism)
• Dexamethasone (therapeutic use)
• Disease Models, Animal
• Eosine Yellowish-(YS) (therapeutic use)
• Glutathione Peroxidase
• Hematoxylin (therapeutic use)
• Interleukin-13
• Interleukin-4
• Interleukin-5
• Luteolin (therapeutic use)
• Malondialdehyde
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• NF-E2-Related Factor 2 (metabolism)
• NF-KappaB Inhibitor alpha
• NF-kappa B (metabolism)
• Ovalbumin (adverse effects)
• Reactive Oxygen Species
• Superoxide Dismutase