Deciphering cilia and ciliopathies using proteomic approaches.

Abstract

Cilia are microtubule-based organelles that protrude from the cell surface and play crucial roles in cellular signaling pathways and extracellular fluid movement. Defects in the ciliary structures and functions are implicated in a set of hereditary disorders, including polycystic kidney disease, nephronophthisis, and Bardet-Biedl syndrome, which are collectively termed as ciliopathies. The application of mass spectrometry-based proteomic approaches to explore ciliary components provides important clues for understanding their physiological and pathological roles. In this review, we focus primarily on proteomic studies involving the identification of proteins in motile cilia and primary cilia, proteomes in ciliopathies, and interactomes of ciliopathy proteins. Collectively, the integration of these data sets will be beneficial for the comprehensive understanding of ciliary structures and exploring potential biomarkers and therapeutic targets for ciliopathies.

• Authors: Xiying Chen, Zhouyuanjing Shi, Feng Yang, Tianhua Zhou, Shanshan Xie
• Journal: The FEBS journal (FEBS J) Vol. 290 Issue 10 Pg. 2590-2603 (05 2023) ISSN: 1742-4658 [Electronic] England
• PMID: 35633520 (Publication Type: Journal Article, Review, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Topics

• Humans
• Proteomics
• Cilia (metabolism)
• Ciliopathies (genetics)
• Polycystic Kidney Diseases (metabolism)