Grifola frondosa Polysaccharide Ameliorates Early Diabetic Nephropathy by Suppressing the TLR4/NF-κB Pathway.

Abstract

Grifola frondosa is a medicinal macro-fungus with a wide range of biological activities. Polysaccharides from Grifola frondosa (PGF) play a positive role in regulating blood glucose and alleviating kidney injury. Here, we investigated the exact mechanism of action by which PGF ameliorates diabetic nephropathy. Our results showed that PGF effectively improved glucose tolerance and insulin sensitivity in streptozocin (STZ)-induced DN mice. Additionally, administration of PGF also ameliorated renal function and inflammatory response in STZ-induced DN mice. Consistent with the in vitro results, the high glucose-induced inflammatory response and apoptosis of renal tubular epithelial cells were decreased by PGF treatment. Furthermore, PGF not only suppressed the expression of TLR4, but also more effectively protected the kidney and reduced the inflammatory response when TLR4 was inhibited. All these data revealed that PGF alleviates diabetic nephropathy by blocking the TLR4/NF-κB pathway.

• Authors: Tao Jiang, Sile Shen, Liang Wang, Mingzhi Zhao, Yangyan Li, Shuting Huang
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 194 Issue 9 Pg. 4093-4104 (Sep 2022) ISSN: 1559-0291 [Electronic] United States
• PMID: 35616773 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Chemical References

• Blood Glucose
• NF-kappa B
• Polysaccharides
• Streptozocin
• Tlr4 protein, mouse
• Toll-Like Receptor 4

Topics

• Animals
• Mice
• Blood Glucose (metabolism)
• Diabetes Mellitus, Experimental (metabolism)
• Diabetic Nephropathies (drug therapy)
• Grifola (metabolism)
• NF-kappa B (metabolism)
• Polysaccharides (pharmacology, therapeutic use)
• Streptozocin (adverse effects)
• Toll-Like Receptor 4 (metabolism)