Abstract |
Given the clinical, economic, and societal impact of obesity, unraveling the mechanisms of adipose tissue expansion remains of fundamental significance. We previously showed that white adipose tissue (WAT) levels of 3-mercaptopyruvate sulfurtransferase (MPST), a mitochondrial cysteine-catabolizing enzyme that yields pyruvate and sulfide species, are downregulated in obesity. Here, we report that Mpst deletion results in fat accumulation in mice fed a high-fat diet (HFD) through transcriptional and metabolic maladaptation. Mpst-deficient mice on HFD exhibit increased body weight and inguinal WAT mass, reduced metabolic rate, and impaired glucose/ insulin tolerance. At the molecular level, Mpst ablation activates HIF1α, downregulates subunits of the translocase of outer/inner membrane (TIM/ TOM) complex, and impairs mitochondrial protein import. MPST deficiency suppresses the TCA cycle, oxidative phosphorylation, and fatty acid oxidation, enhancing lipid accumulation. Sulfide donor administration to obese mice reverses the HFD-induced changes. These findings reveal the significance of MPST for white adipose tissue biology and metabolic health and identify a potential new therapeutic target for obesity.
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Authors | Antonia Katsouda, Dimitrios Valakos, Vasilios S Dionellis, Sofia-Iris Bibli, Ioannis Akoumianakis, Sevasti Karaliota, Karim Zuhra, Ingrid Fleming, Noriyuki Nagahara, Sophia Havaki, Vassilis G Gorgoulis, Dimitris Thanos, Charalambos Antoniades, Csaba Szabo, Andreas Papapetropoulos |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 219
Issue 7
(07 04 2022)
ISSN: 1540-9538 [Electronic] United States |
PMID | 35616614
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022 Katsouda et al. |
Chemical References |
- Mitochondrial Proteins
- Sulfides
- Sulfurtransferases
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Topics |
- Animals
- Diet, High-Fat
- Energy Metabolism
- Glucose Intolerance
- Mice
- Mice, Inbred C57BL
- Mitochondrial Proteins
(metabolism)
- Obesity
(metabolism)
- Sulfides
- Sulfurtransferases
(metabolism)
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