Exosome-mediated aptamer S58 reduces fibrosis in a rat glaucoma filtration surgery model.

Abstract

AIM:

To confirm whether exosome-mediated delivery of aptamer S58 (Exo-S58) has a better antifibrotic effect than naked S58 in human conjunctival fibroblasts (HConFs) and a rat glaucoma filtration surgery (GFS) model.

METHODS:

To enhance the effective reaction time of aptamer S58 in vivo, we loaded aptamer S58 into exosomes derived from HEK293T cells by PEI transfection to determine the effect of Exo-S58 in HConFs and a rat GFS model.

RESULTS:

Exo-S58 can significantly reduce cell proliferation, migration and fibrosis in TGF-β2-induced HConFs. In an in vivo experiment, Exo-S58 treatment prolonged filtering bleb retention and reduced fibrosis compared with naked S58 treatment in GFS rats.

CONCLUSION:

The exosomes are safe and valid carriers to deliver aptamers. Furthermore, Exo-S58 exhibited superior antifibrotic effect than naked S58 both in HConFs cells and rat GFS models.

• Authors: Qian-Yi Lin, Xiang-Ji Li, Yu Leng, Xiao-Min Zhu, Min Tang, Yi Lin, Wang-Du Luo, Bing-Cai Jiang, Xia Chen, Lin Xie
• Journal: International journal of ophthalmology (Int J Ophthalmol) Vol. 15 Issue 5 Pg. 690-700 ( 2022) ISSN: 2222-3959 [Print] China
• PMID: 35601180 (Publication Type: Journal Article)
• Copyright: International Journal of Ophthalmology Press.