Long non-coding RNA CYTOR modulates cancer progression through miR-136-5p/MAT2B axis in renal cell carcinoma.

Abstract

BACKGROUND:

To explore the role of long noncoding RNAs (lncRNAs) cytoskeleton regulator RNA (CYTOR) in renal cell carcinoma (RCC).

METHODS:

The levels of CYTOR in RCC tissues and cell lines were detected by RT-qPCR. 786-O and Caki-1 cells were transfected with CYTOR-shRNA or pcDNA-CYTOR respectively, or co-transfected with CYTOR-shRNA and miR-136-5p inhibitor, or co-transfected with miR-136-5p mimic and pcDNA-MAT2B. MTT assay, Transwell assay and flow cytometry were used to evaluate cell proliferation, invasion and apoptosis. The relationship between lncRNA CYTOR and miRNA-136-5p was detected by dual luciferase reporter gene and RNA pull down assays, and the targeted relationship between miRNA-136-5p and MAT2B was verified by dual luciferase reporter gene assay. The interaction between MAT2B and BAG3 protein was verified by co-IP experiment. The role of lncRNA CYTOR in vivo was also examined.

RESULTS:

LncRNA CYTOR was up-regulated in RCC tissues and cell lines, and miR-136-5p was down-regulated in renal carcinoma cell lines and tissues. Downregulation of CYTOR inhibited cell proliferation and invasion and promoted apoptosis. miR-136-5p was sponged by lncRNA CYTOR, which negatively regulated the development of RCC. MAT2B was a target gene of miR-136-5p. MAT2B protein interacted directly with BAG3 protein to affect the proliferation, invasion and apoptosis of RCC cells. In vivo experiments showed that the expression level of miR-136-5p was increased, and MAT2B expression was decreased after CYTOR knockdown, thereby inhibiting the development of RCC.

CONCLUSIONS:

LncRNA CYTOR promoted the progression of RCC by targeting miR-136-5p to regulate the target gene MAT2B, which interacted with BAG3 protein.

• Authors: Dan Wang, Xiaojun Zhu, Buhe Siqin, Chao Ren, Faxian Yi
• Journal: Toxicology and applied pharmacology (Toxicol Appl Pharmacol) Vol. 447 Pg. 116067 (07 15 2022) ISSN: 1096-0333 [Electronic] United States
• PMID: 35597301 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022. Published by Elsevier Inc.

Chemical References

• Adaptor Proteins, Signal Transducing
• Apoptosis Regulatory Proteins
• BAG3 protein, human
• MIRN136 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• RNA, Small Interfering
• MAT2B protein, human
• Methionine Adenosyltransferase

Topics

• Adaptor Proteins, Signal Transducing (metabolism)
• Apoptosis Regulatory Proteins (metabolism)
• Carcinoma, Renal Cell (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cytoskeleton
• Gene Expression Regulation, Neoplastic
• Humans
• Kidney Neoplasms (genetics)
• Methionine Adenosyltransferase (metabolism)
• MicroRNAs (genetics, metabolism)
• RNA, Long Noncoding (genetics, metabolism)
• RNA, Small Interfering