Melanoma spheroid-containing artificial dermis as an alternative approach to in vivo models.

Abstract

Melanoma spheroid-loaded 3D skin models allow for the study of crucial tumor characteristics and factors at a superior level because the neoplastic cells are integrated into essential human skin components, permitting tumor-skin model communication. Herein, we designed a melanoma-containing artificial dermis by inserting multicellular tumor spheroids from the metastatic phase of WM 1617 melanoma cells into an artificial dermis. We cultured multicellular melanoma spheroids by hanging drop method (250 cells per drop) with a size of 420 μm in diameter after incubation for 14 days. These spheroids were integrated into the dermal equivalents that had been previously preparedwith a type-I collagen matrix and healthy fibroblasts. The melanoma spheroid cells invaded and proliferated in the artificial dermis. Spheroids treated with a 1.0 μmol/L aluminum chloride phthalocyanine nanoemulsion in the absence of light showed high cell viability. In contrast, under irradiation with visible red light (660 nm) at 25 J/cm2, melanoma cells were killed and the healthy tissue was preserved, indicating that photodynamic therapy is effective in such a model. Therefore, the 3D skin melanoma model has potential to promote research in full-thickness skin model targeting optimized preclinical assays.

• Authors: Danielli Azevedo Monico, Italo Rodrigo Calori, Carla Souza, Enilza Maria Espreafico, Hong Bi, Antonio Claudio Tedesco
• Journal: Experimental cell research (Exp Cell Res) Vol. 417 Issue 1 Pg. 113207 (08 01 2022) ISSN: 1090-2422 [Electronic] United States
• PMID: 35580698 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Topics

• Dermis
• Humans
• Melanoma
• Skin Neoplasms
• Spheroids, Cellular