Protective effects of Radix Isatidis polysaccharide ameliorates obesity via promotion AMPK pathway in high-fat-diet-induced obese rats and 3T3-L1 adipocyte cells.

Abstract

OBJECTIVES:

The purpose of this paper is to ascertain the effect and mechanism of Radix Isatidis polysaccharide (RIP) on obesity.

METHODS:

High fat diet (HFD)-induced obese rats and the MDI-induced 3T3-L1 adipocyte cells were established to evaluate the ameliorated obesity effect and mechanism from RIP.

KEY FINDINGS:

Experiments in vivo show that oral administration of RIP has significant preventive effects on HFD-induced obesity and metabolic disorders in rats. With treatment of RIP (20, 40 and 80 mg/kg BW), the body weight, fat accumulation, adipocyte cell size, serum lipid levels and antioxidant enzyme activity were progressively improved. On the other hand, the treatment of 3T3-L1 cells with RIP (25, 50 and 100 mg/L) led to a decrease in lipid accumulation and glucose consumption. In addition, during adipogenesis in 3T3-L1 cells, RIP remarkably down-regulated mRNA levels of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα), sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS), acetyl-CoA carboxylase and glycerol-3-phosphate dehydrogenase. Furthermore, after RIP treatment, the protein expression of PPARγ, C/EBPα, FAS, HMG-CoA reductase and acetyl-CoA synthetase-1 (AceCS1) were significantly decreased and the expression of p-AMPK was increased.

CONCLUSION:

These results highlight the potential of RIP for obesity interventions and suggest that RIP inhibited adipocyte differentiation and lipid synthesis by activating adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signalling pathway and down-regulating the expression of major adipogenic transcription factors, PPARγ, C/EBPα, etc.

• Authors: Ye Yuan, Dong-Ni Wang, Wen-You Zhang, Ting-Ting Sun, Yi-Ting Feng, Mo-Xiang Liu, Ji-Ping Li
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 74 Issue 8 Pg. 1170-1179 (Aug 19 2022) ISSN: 2042-7158 [Electronic] England
• PMID: 35579414 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2022. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Anti-Obesity Agents
• CCAAT-Enhancer-Binding Protein-alpha
• Drugs, Chinese Herbal
• Lipids
• PPAR gamma
• Polysaccharides
• banlangen
• AMP-Activated Protein Kinases

Topics

• 3T3-L1 Cells
• AMP-Activated Protein Kinases (metabolism)
• Adipocytes (metabolism)
• Adipogenesis
• Animals
• Anti-Obesity Agents (pharmacology)
• Body Weight
• CCAAT-Enhancer-Binding Protein-alpha (metabolism)
• Diet, High-Fat (adverse effects)
• Drugs, Chinese Herbal
• Lipids
• Mice
• Obesity (drug therapy, metabolism, prevention & control)
• PPAR gamma (metabolism)
• Polysaccharides (pharmacology)
• Rats