CircRNA has_circ_0069313 induced OSCC immunity escape by miR-325-3p-Foxp3 axes in both OSCC cells and Treg cells.

Abstract

INTRODUCTION:

CircRNAs are engaged in the tumorigenesis and progression of oral squamous cancer cells (OSCC). However, the function and underlying mechanism of circRNAs on tumor-associated immunity escape are largely unknown.

MATERIALS AND METHODS:

We analyzed the expression pattern of has_circ_0069313 in our in-house database and its correlation with OSCC prognosis. Immunohistochemistry was applied to detected PDL1 expression. RNA fluorescence in situ hybridization was applied to detect subcellular location of circRNA. A luciferase activity assay was used to detect the interaction of has_circ_0069313 and miR-325-3p and its downstream target, Foxp3. Exosomes were collected to detect the exosomal circRNAs and co-culture assays were performed to detect the function of exosomal circRNAs on Tregs.

RESULTS:

has_circ_0069313 was upregulated in OSCC tissues and predicts poor prognosis. has_circ_0069313 promotes immunity escape through inhibiting miR-325-3p-induced Foxp3 degradation. has_circ_0069313 is an exosomal circRNA and the transfer of has_circ_0069313 to Treg cells promotes the Treg function through maintaining Foxp3 levels.

CONCLUSION:

Our results indicate that has_circ_0069313 induces OSCC immunity escape via the miR-325-3p-Foxp3 axis in both OSCC cells and Treg cells.

• Authors: Yiyang Chen, Zeyu Li, Jianfeng Liang, Jiayu Liu, Jiansuo Hao, Quan Wan, Jiameng Liu, Chongdai Luo, Zhiyuan Lu
• Journal: Aging (Aging (Albany NY)) Vol. 14 Issue 10 Pg. 4376-4389 (05 16 2022) ISSN: 1945-4589 [Electronic] United States
• PMID: 35575762 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• FOXP3 protein, human
• Forkhead Transcription Factors
• MIRN325 microRNA, human
• MicroRNAs
• RNA, Circular
• Transcription Factors

Topics

• Cell Proliferation
• Forkhead Transcription Factors (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• In Situ Hybridization, Fluorescence
• MicroRNAs (genetics, metabolism)
• Mouth Neoplasms (metabolism)
• RNA, Circular (genetics)
• T-Lymphocytes, Regulatory (metabolism)
• Transcription Factors (metabolism)