Abstract | Background: Methods: Quantitative real-time PCR (qRT-PCR) was used to detect SNHG3 and miR-448 in gastric cancer, and a dual- luciferase experiment verified the effects of SNHG3, miR-448, and DNMT1. After abnormally expressing SNHG3, miR-448, and DNMT1 alone or together, methylation-specific PCR was performed to determine the methylation of SEPT9, Western blotting was performed to detect the expression of DNA methyltransferase 1 (DNMT1) and SEPT9, and Transwell, scratch, and CCK-8 assays were performed to reveal the invasion, migration, and cell growth of gastric cancer cells. Results: We found that SNHG3 was upregulated in gastric cancer and that SNHG3 knockdown or miR-448 overexpression inhibited SEP9 methylation and therefore increased its expression, thereby inhibiting the growth, metastasis, and spread of gastric cancer cells. Conclusion: Our study indicates that SNHG3 regulates SEPT9 methylation by targeting miR-448/DNMT1 and subsequently affecting the occurrence and development of gastric cancer.
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Authors | Wei Li, Xudong Ma, Feng Wang, Shi Chen, Qingxiong Guo, Feng Sun, Yongqing Duan |
Journal | Journal of oncology
(J Oncol)
Vol. 2022
Pg. 3433406
( 2022)
ISSN: 1687-8450 [Print] Egypt |
PMID | 35528235
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Wei Li et al. |