Construction of K+ responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes.

Abstract

Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer, we fabricated 2-O-α-d-glucopyranosyl-l-ascorbic acid (AA-2G)-loaded polycaprolactone (PCL)-crown ether micro/nanofibers on the surface of styrene-b-(ethylene-co-butylene)-b-styrene (SEBS). Our strategy is based on the sensitive response of the crown ether to leaked potassium, causing the release of AA-2G, the AA-2G can then remove the excess ROS, maintaining the Na/K-pump activity and the cell integrity. We demonstrated that the PCL-crown ether micro/nanofibers have been well prepared on the surface of SEBS; the micro/nanofibers provide a sensitive response to excess K+ and trigger the rapid release of AA-2G. AA-2G then acts as an antioxidant to reduce the excess ROS and maintain the Na/K-pump activity to mitigate cation leaks, resulting in the reduced hemolysis of the preserved erythrocytes. Our work thus provides a novel method for the development of plasticizer-free polymers for the storage of erythrocytes, and has the potential to be used to fabricate long-term anti-hemolytic biomaterials for in vivo use.

• Authors: Xingkun Luan, Haozheng Wang, Zehong Xiang, Jiruo Zhao, Ying Feng, Qiang Shi, Baijun Liu, Yumei Gong, Shing-Chung Wong, Jinghua Yin
• Journal: RSC advances (RSC Adv) Vol. 9 Issue 10 Pg. 5251-5258 (Feb 11 2019) ISSN: 2046-2069 [Electronic] England
• PMID: 35515950 (Publication Type: Journal Article)
• Copyright: This journal is © The Royal Society of Chemistry.