Lysine acetylation (Kac) on
histone promotes relaxation of the
chromatin conformation and favors gene transcription to regulate
oncogenesis, whereas the total acetylation profiling of
oral squamous cell carcinoma (OSCC) is unknown. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilised to investigate
lysine acetylation features of
tumor tissues and adjacent normal tissues from 9 patients with OCSS. 282 upregulated Kac sites in 234
proteins and 235 downregulated Kac sites in 162
proteins between OSCC tissues and paired adjacent normal tissues were identified. Different acetylation
proteins (
DAPs) were analyzed through KEGG-based and MCODE. These
DAPs are enriched in the ribosome biogenesis pathway. Survival Analysis of hub genes with TCGA database was performed. In addition, IPA software was used to explore the connection between 9 core
DAPs (RPS3, RPL24, RPL19, EIF4A2, RPL12, MYBPC1, RPS6, ARCN1, and TMEM9) and the different expression of KATs and KDACs identified in our proteomic. The study is the first comparative study of Kac modification on
oral squamous cell carcinoma. We propose to put forward the hypothesis that the dysfunction of ribosome biogenesis caused by the change of
Lysine acetylation, especially downregulated acetylation on RPS6 and RPS3 may associated with the pathogenesis of OSCC. SIGNIFICANCE: The study is the first comparative study of Kac modification on
oral squamous cell carcinoma through LC-MS/MS-based modified proteomic. These
DAPs are high enriched in the ribosome biogenesis pathway. Used MCODE and survival analysis, 9 core
DAPs (RPS3, RPL24, RPL19, EIF4A2, RPL12, MYBPC1, RPS6, ARCN1, and TMEM9) were screened. IPA software was used to explore the connection between 9 core
DAPs and the different expression of KATs and KDACs identified in our proteomic. In addition, we propose to put forward the hypothesis that the dysfunction of ribosome biogenesis caused by the change of
Lysine acetylation, especially downregulated acetylation on RPS6 and RPS3 may associated with the pathogenesis of OSCC.