Abstract | BACKGROUND: METHODS: We collected peripheral CD4+ T cells from 12 GD patients, including 6 newly diagnosed GD (NGD) and 6 refractory GD (RGD) patients, 6 HT patients and 6 healthy controls, and examined the gene expression profiles and colon types of T cells receptor (TCR) β chain complementarity determining region 3 (CDR3) using high-throughput sequencing. RESULTS: The TCR repertoire were significantly expanded in AITDs groups, and some TCR genes were expressed more preferentially in AITDs group than in the healthy control group, including TRBV15 (P = 0.001), TRBV4-2 (P = 0.003), TRBV9 (P = 0.007), TRBV3-2 (P = 0.012), TRBV7-8 (P = 0.015), TRBV25-1 (P = 0.019), TRBV12-4 (P = 0.019) and TRBV27 (P = 0.02) in GD patients as well as TRBV29-1 (P = 0.004), TRBV12-4 (P = 0.004), TRBV6-5 (P = 0.011), TRBV7-2 (P = 0.012), TRBV27 (P = 0.012), TRBV9 (P = 0.031) and TRBV4-2 (P = 0.032) in HT patients. Moreover, subgroup analysis showed that the difference in the TCR spectrum between the normal group and NGD was not obvious, but a large number of differential genes appeared in the RGD group. CONCLUSION: TCR spectrum has changed in patients with AITDs with expanded repertoire and many upregulated TRBV genes. Moreover, this difference is not apparent in GD patients at the initial stage, but as the disease progresses, the differences in TCR profiles became more pronounced.
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Authors | Xi Jia, Tian-Yu Zhai, Bing Wang, Jin-An Zhang, Rong-Hua Song |
Journal | Molecular and cellular endocrinology
(Mol Cell Endocrinol)
Vol. 550
Pg. 111644
(06 15 2022)
ISSN: 1872-8057 [Electronic] Ireland |
PMID | 35429598
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Oligopeptides
- Receptors, Antigen, T-Cell
|
Topics |
- Autoimmune Diseases
(genetics)
- Graves Disease
(genetics, pathology)
- Hashimoto Disease
(genetics)
- High-Throughput Nucleotide Sequencing
- Humans
- Oligopeptides
(genetics)
- Receptors, Antigen, T-Cell
(genetics)
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