Abstract | BACKGROUND: RESULTS: We perform single-cell RNA sequencing analysis of 19 surgical tissue specimens from 8 clear cell renal cell carcinoma (ccRCC) patients with tumor thrombus. We observe tumor thrombus has increased tissue resident CD8+ T cells with a progenitor exhausted phenotype compared with the matched primary tumors. Remarkably, macrophages, malignant cells, endothelial cells and myofibroblasts from TTs exhibit enhanced remodeling of the extracellular matrix. The macrophages and malignant cells from primary tumors represent proinflammatory states, but also increase the expression of immunosuppressive markers compared to tumor thrombus. Finally, differential gene expression and interaction analyses reveal that tumor-stroma interplay reshapes the extracellular matrix in tumor thrombus associated with poor survival. CONCLUSIONS:
|
Authors | Yue Shi, Qi Zhang, Hai Bi, Min Lu, Yezhen Tan, Daojia Zou, Liyuan Ge, Zhigang Chen, Cheng Liu, Weimin Ci, Lulin Ma |
Journal | Genome biology
(Genome Biol)
Vol. 23
Issue 1
Pg. 87
(03 31 2022)
ISSN: 1474-760X [Electronic] England |
PMID | 35361264
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2022. The Author(s). |
Topics |
- CD8-Positive T-Lymphocytes
- Carcinoma, Renal Cell
(complications, genetics)
- Ecosystem
- Endothelial Cells
- Humans
- Kidney Neoplasms
(complications, genetics, pathology)
- Nephrectomy
- Sequence Analysis, RNA
- Thrombosis
(complications, pathology, surgery)
- Vena Cava, Inferior
(pathology, surgery)
|