Generation of induced pluripotent stem cell line derived from FNAIT patient with CD36 deficiency mutations.

Abstract

Fetal and neonatal alloimmune thrombocytopenia (FNAIT) caused by anti-CD36 isoantibodies is a common disease and the frequency of type I CD36 deficiency is relatively high in eastern Asian populations.Currently, patient-specific induced pluripotent stem cells (hiPSC) are believed to be useful tools for studying anti-CD36 mediated FNAIT and finding new therapeutic approaches to the disease.We generated an iPSC line from peripheral blood mononuclear cells of a patient carrying a 329-330delAC of the CD36 gene.The iPSC expressed pluripotency markers, gave rise to derivatives of three germ layers during spontaneous differentiation, had a normal karyotype, and retained the patient-specific mutation.

• Authors: Dawei Chen, Tao Wang, Xin Ye, Xiuzhang Xu, Wenjie Xia, Yongshui Fu
• Journal: Stem cell research (Stem Cell Res) Vol. 61 Pg. 102749 (05 2022) ISSN: 1876-7753 [Electronic] England
• PMID: 35305469 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Topics

• Blood Platelet Disorders
• Cell Line
• Genetic Diseases, Inborn
• Humans
• Induced Pluripotent Stem Cells (metabolism)
• Infant, Newborn
• Leukocytes, Mononuclear
• Mutation (genetics)
• Thrombocytopenia, Neonatal Alloimmune (metabolism)