TDP-43 is mislocalized from the nucleus and aggregates within the cytoplasm of affected neurons in cases of
amyotrophic lateral sclerosis. TDP-43 pathology has also been found in brain tissues under non-
amyotrophic lateral sclerosis conditions, suggesting mechanistic links between TDP-43-related
amyotrophic lateral sclerosis and various
neurological disorders. This study aimed to assess TDP-43 pathology in the spinal cord motor neurons of
tauopathies. We examined 106 spinal cords from consecutively autopsied cases with
progressive supranuclear palsy (n = 26),
corticobasal degeneration (n = 12), globular glial
tauopathy (n = 5),
Alzheimer's disease (n = 21) or
Pick's disease (n = 6) and neurologically healthy controls (n = 36). Ten of the
progressive supranuclear palsy cases (38%) and seven of the
corticobasal degeneration cases (58%) showed mislocalization and cytoplasmic aggregation of TDP-43 in spinal cord motor neurons, which was prominent in the cervical cord. TDP-43 aggregates were found to be skein-like, round-shaped, granular or dot-like and contained insoluble C-terminal fragments showing blotting pattern of
amyotrophic lateral sclerosis or
frontotemporal lobar degeneration. The lower motor neurons also showed
cystatin-C aggregates, although Bunina bodies were absent in haematoxylin-
eosin staining. The spinal cord TDP-43 pathology was often associated with TDP-43 pathology of the primary motor cortex. Positive correlations were shown between the severities of TDP-43 and four-repeat (4R)-tau aggregates in the cervical cord. TDP-43 and 4R-tau aggregates burdens positively correlated with microglial burden in anterior horn. TDP-43 pathology of spinal cord motor neuron did not develop in an age-dependent manner and was not found in the
Alzheimer's disease,
Pick's disease, globular glial
tauopathy and control groups. Next, we assessed SFPQ expression in spinal cord motor neurons; SFPQ is a recently identified regulator of
amyotrophic lateral sclerosis/
frontotemporal lobar degeneration pathogenesis, and it is also reported that interaction between SFPQ and FUS regulates splicing of MAPT exon 10. Immunofluorescent and proximity-
ligation assays revealed altered SFPQ/FUS-interactions in the neuronal nuclei of
progressive supranuclear palsy,
corticobasal degeneration and
amyotrophic lateral sclerosis-TDP cases but not in
Alzheimer's disease,
Pick's disease and globular glial
tauopathy cases. Moreover, SFPQ expression was depleted in neurons containing TDP-43 or 4R-tau aggregates of
progressive supranuclear palsy and
corticobasal degeneration cases. Our results indicate that
progressive supranuclear palsy and
corticobasal degeneration may have properties of systematic motor neuron
TDP-43 proteinopathy, suggesting mechanistic links with
amyotrophic lateral sclerosis-TDP. SFPQ dysfunction, arising from altered interaction with FUS, may be a candidate of the common pathway.