Effects of exposure to oxytetracycline on the liver proteome of red seabream (Pagrus major) in a real administration scenario.

Abstract

Oxytetracycline (OTC) is a widely used antibiotic in aquaculture. In this study, red seabream (Pagrus major), the most popular aquaculture species in Japan, were treated with OTC mimicking a real administration scenario in aquaculture. The treatment groups were as follows: no OTC, 40 mg/kg body wt/day (equivalent to the dose used in actual aquaculture), or 178 mg/kg body wt/day. The first exposure was conducted for a week (1st OTC exposure period), followed by a 4-week interval, and the second exposure was for one week (2nd OTC exposure period). We investigated the effects of OTC on the liver proteome with the isobaric tags for relative and absolute quantitation (iTRAQ) technology accompanied by liquid chromatography and mass spectrometry. The pathway and disease enrichment analyses of differentially abundant proteins in OTC-exposed groups compared to their respective controls showed that the abundance of proteins related to the immune and nervous systems was altered after the 1st and 2nd OTC exposures, respectively. Quantitative real-time PCR of the transcripts of immune-related genes corroborated with the results of proteome analysis. OTC exposure also modulated the expression of metabolism-related proteins after the 1st and 2nd OTC exposures. Furthermore, after four weeks of the 2nd exposure, weight loss and changes in the expression of proteins related to metabolism were observed, suggesting that OTC exposure disrupts the metabolic system and causes growth inhibition. Based on these results, we suggest that the use of OTC in aquaculture poses a health risk in fish species. Thus, we need to pay more attention to the contamination with OTC in aquaculture.

• Authors: Midori Iida, Hoa Thanh Nguyen, Fumiya Takahashi, Su-Min Bak, Kazuki Kanda, Hisato Iwata
• Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (Comp Biochem Physiol C Toxicol Pharmacol) Vol. 256 Pg. 109325 (Jun 2022) ISSN: 1532-0456 [Print] United States
• PMID: 35272040 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Proteome
• Oxytetracycline

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Liver
• Oxytetracycline (toxicity)
• Proteome
• Sea Bream