Glioma is one of the most common intracranial
tumors worldwide, and
metastasis and chemoresistance remain a challenge in
glioma treatment. This study aims to investigate the effect of
sodium valproate on the invasion and
metastasis of
glioma cells and its mechanism.
Glioma cell lines were stimulated with VPA at different concentrations and for different durations of action. U87
glioma cells were transfected with Smad4 plasmid and
small interfering RNA, and the changes of EMT-related
protein indexes in U87 cells after up- or downregulation of Smad4 were detected by Western blotting. Immunohistochemistry was used to detect the differences in the expression of Smad4, TIF1-γ, and TGF-β
proteins in 39
glioma clinical specimens from the Department of Pathology of our hospital. Based on the regulation of EMT-related
transcription factors by VPA, our study indicates that VPA inhibits the EMT process of
glioma by altering the expression level of Smad4, which is induced by TGF-β1 to form a Smad3/4 complex, thus inducing the EMT process of the
tumor and acting as an antitumor target to inhibit the invasive ability of
glioma cells.
Sodium valproate inhibits
glioma invasion and
metastasis through the regulation of Smad4 expression.