[Electroacupuncture of acupoints of heart meridian ameliorates acute myocardial ischemia via Akt/mTOR pathway].

Abstract

OBJECTIVE:

To observe the effect of electroacupuncture (EA) on the expression of myocardial protein kinase B (Akt) and mammalian target of rapamycin (mTOR) in acute myocardial ischemia (AMI) rats.

METHODS:

Thirty male SD rats were randomly divided into control, model and EA groups (n=10 in each group). The AMI model was established by occlusion of the descending anterior branch (DAB) of the left coronary artery. EA (2 Hz, 1-2 mA) was applied to bilateral "Shenmen" (HT7) and "Tongli" (HT5) for 20 min, once daily for consecutive 7 days. The electrocardiogram (ECG) of nape-xiphoid lead was recorded for assessing changes of myocardial ischemia. Histopathologic changes of the ischemic myocardial tissue were observed after H.E. staining and ultra-microstructural changes of cardiomyocytes observed by transmission electron microscopy (TEM). The expression levels of Akt, phosphorylated-Akt (p-Akt), mTOR and phosphorylated-mTOR (p-mTOR) in the myocardium were detected by Western blot, followed by calculating the ratios of p-Akt/Akt and p-mTOR/mTOR.

RESULTS:

Following ligature of DAB, the ECG-ST level was significantly increased in the model group in comparison with the control group (P<0.01). At 30 min after treatment, the ECG-ST level decreased significantly compared with the model group (P<0.01). At the end of the 7-day treatment period, the ECG-ST level increased compared with the model group (P<0.05). The levels of myocardial p-Akt and p-mTOR protein expression, and the ratios of p-Akt/Akt and p-mTOR/mTOR were significantly lower in the model group than those in the control group (P<0.01), and considerably increased in the EA group than in the model group (P<0.01). No significant differences were found among the three groups in the expression levels of Akt and mTOR proteins (P>0.05). Outcomes of H.E. staining and TEM showed damage of mitochondria and occurrence of a large number of autophagosomes in myocardiocytes in the model group, which was milder in the EA group.

CONCLUSION:

EA at HT5 and HT7 can improve AMI in AMI rats, which may be related to its effect in facilitating Akt/mTOR signaling.

• Authors: Li-Bin Wu, Fan Zhang, Qing Yu, Min-Jun Wang, Zhi-Ming Jiang, Li-Na Zhao, Jie Wang, Rong-Lin Cai, Zi-Jian Wu, Ling Hu
• Journal: Zhen ci yan jiu = Acupuncture research (Zhen Ci Yan Jiu) Vol. 47 Issue 2 Pg. 121-8 (Feb 25 2022) ISSN: 1000-0607 [Print] China
• PMID: 35218621 (Publication Type: Journal Article)

Chemical References

• mTOR protein, rat
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases

Topics

• Acupuncture Points
• Animals
• Electroacupuncture
• Male
• Meridians
• Myocardial Ischemia (genetics, therapy)
• Myocytes, Cardiac
• Proto-Oncogene Proteins c-akt (genetics)
• Rats
• Rats, Sprague-Dawley
• TOR Serine-Threonine Kinases (genetics)