Proximity Ligation Mapping of Microcephaly Associated SMPD4 Shows Association with Components of the Nuclear Pore Membrane.

Abstract

SMPD4 is a neutral sphingomyelinase implicated in a specific type of congenital microcephaly. Although not intensively studied, SMPD4 deficiency has also been found to cause cell division defects. This suggests a role for SMPD4 in cell-cycle and differentiation. In order to explore this role, we used proximity ligation to identify the partners of SMPD4 in vivo in HEK293T cells. We found that these partners localize near the endoplasmic reticulum (ER) and the nuclear membrane. Using mass spectrometry, we could identify these partners and discovered that SMPD4 is closely associated with several nucleoporins, including NUP35, a nucleoporin directly involved in pore membrane curvature and pore insertion. This suggests that SMPD4 may play a role in this process.

• Authors: Alexandra C A Piët, Marco Post, Dick Dekkers, Jeroen A A Demmers, Maarten Fornerod
• Journal: Cells (Cells) Vol. 11 Issue 4 (02 15 2022) ISSN: 2073-4409 [Electronic] Switzerland
• PMID: 35203325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nuclear Pore Complex Proteins
• Sphingomyelin Phosphodiesterase

Topics

• HEK293 Cells
• Humans
• Microcephaly (metabolism)
• Nuclear Envelope (metabolism)
• Nuclear Pore (metabolism)
• Nuclear Pore Complex Proteins (metabolism)
• Sphingomyelin Phosphodiesterase (metabolism)