The survival reduction after
transplantation limited the clinical uses of stem cells so the current study explored preconditioning adipose-derived stem cells (ADMSCs) and
all-trans retinoic acid (ATRA) effects on
cisplatin that caused
acute kidney injury (AKI). One hundred and fifty Sprague-Dawley male rats were distributed into five groups: control group;
Cisplatin (CIS) group; CIS and ATRA group; CIS and ADMSC group, and CIS, ATRA, and ADMSCs group. Ten rats were euthanized after 3rd, 7th, and 11th days from CIS injection. Renal function, molecular studies, and histopathological analysis were studied. The preconditioning of ADMSCs with ATRA increased the viability of the cells which was reflected in the amelioration of kidney functions after CIS injection by the significant reduction of serum
creatinine, microalbuminuria, as well as NO, and the significant rise of
creatinine clearance, as well as SOD compared to the group of
cisplatin. ATRA also supported ADMSCs by a significant down-regulation of
caspase-3,
il-6 and TGFβ1, and a significant up-regulation of HIF1,
VEGF and CD31 compared to group of
cisplatin which reversed the
cisplatin effect. ATRA increased renoprotective properties of ADMSCs against
cisplatin- induced AKI by reducing the apoptosis,
inflammation, and stimulating angiogenesis.